Video Channel of China Wuhan Desheng Biochemical Technology Co., Ltd
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View The influence of temperature and pH value on the use of IVD diagnostic enzymes Demo

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November 28, 2025
Category Connection: Ενζυμική προετοιμασία
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Tags:
#glutamate δοκιμή διυδρογενάσεων #proteolytic ενζυμικά συμπληρώματα #δοκιμή οξειδάσεων χοληστερόλης
Brief: Watch the demo to catch practical tips and quick performance insights on how temperature and pH value influence IVD diagnostic enzymes. This video showcases how these environmental factors affect enzyme activity and demonstrates strategies for optimizing in-vitro diagnostic test accuracy and reliability.
Related Product Features:
  • Pyruvate kinase is available as a white freeze-dried powder or liquid with a molecular weight of approximately 63 kDa.
  • The enzyme is preserved in 25 mM Tris-HCl buffer at pH 7.50 to maintain stability and activity.
  • Product purity is guaranteed at ≥90% as confirmed by SDS-PAGE detection methods.
  • Requires low-temperature ice pack transport and storage conditions to prevent activity loss from repeated freeze-thaw cycles.
  • Specifically designed for use in in-vitro diagnostic kits to ensure accurate medical testing results.
  • Manufactured by Hubei Xindesheng with professional enzyme preparation research and development expertise.
  • Enzyme activity is highly sensitive to temperature changes, with optimal performance within specific temperature ranges.
  • Catalytic efficiency is significantly affected by pH levels, requiring precise buffering for maximum activity.
FAQS:
  • Why are temperature and pH so important for IVD diagnostic enzymes?
    Temperature and pH are critical environmental factors that directly affect enzyme catalytic activity. Temperature influences molecular movement and substrate binding efficiency, while pH affects the ionization state of active center groups. Both factors must be precisely controlled to ensure accurate and reliable diagnostic test results.
  • What happens when diagnostic enzymes are exposed to incorrect temperature or pH conditions?
    When temperature exceeds the optimal range, enzyme spatial structure can be damaged, leading to decreased or complete loss of activity. Similarly, pH deviations from the optimal range interfere with electrostatic interactions between enzyme and substrate, reducing catalytic efficiency and compromising test accuracy.
  • How can laboratories ensure proper temperature and pH control for diagnostic enzymes?
    Laboratories should use calibrated constant temperature equipment like water baths or incubators, pre-balance reagents and samples at suitable temperatures, employ appropriate biological buffering agents matched to the reaction system, and regularly monitor pH values to maintain stable conditions for optimal enzyme performance.
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