Guidelines for the entire process of separating gel collection tubes and adding gel

In the field of medical testing, the quality of separating gel collection tubes directly affects the serum separation efficiency and detection accuracy. This article will systematically review the entire process of adding glue to the separation glue collection tube, from the amount of glue used, operational skills to special scenario handling, providing practical guidance for production personnel.

Glue dosage and thickness control: precise proportioning is the foundation

The gluing process must strictly follow the corresponding standards for pipe diameter and dosage:

Φ 13mm pipe diameter: It is recommended to add 0.8-1g of adhesive to ensure that the adhesive layer thickness reaches 6mm;

Φ 16mm pipe diameter: It is recommended to add 1.4-1.6g of adhesive, and also ensure a thickness of 6mm.

Insufficient thickness of the adhesive layer can lead to mixing of serum and blood cells, affecting the detection results; Excess may increase production costs. In practical operation, precise control can be achieved through colloid filling equipment. If there are doubts about the dosage, sales personnel can be contacted for personalized solutions.

Winter gluing: temperature management is key

Under low temperature conditions, the separation gel is prone to difficult flipping due to hardening, which affects the quality of serum separation. To address this issue, the following measures need to be taken:

Natural temperature recovery: After transferring the colloid from outdoors to a 25 ℃ laboratory, let it stand for 30 minutes to 1 hour until the colloid softens before operation;

Water bath heating: If rapid heating is required, the colloid can be heated in a 25 ℃ water bath (not exceeding 4 hours) to promote the recovery of colloid fluidity.

By regulating the temperature, it is possible to effectively avoid the problem of poor flipping caused by colloidal hardness, ensuring the efficiency of serum separation.

Laboratory gluing: strategies for addressing wire drawing issues

If there is a wire drawing phenomenon during the gluing process, it not only affects the operational efficiency, but also may contaminate the blood collection tube.

 The solution is as follows:

1. Water bath pretreatment: Heat the colloid in a 25 ℃ water bath (not exceeding 4 hours) to reduce its viscosity;

2. Equipment debugging: Check whether the nozzle of the filling equipment is blocked or worn, and replace the accessories in a timely manner;

3. Operation optimization: Keep the test tube vertical when adding glue, control the filling speed, and avoid excessive friction between the glue and the tube wall.

Static storage and transportation after adding glue: details determine success or failure

After adding the adhesive, it needs to be left vertically for 1-3 minutes to restore the thixotropy of the colloid and form a stable adhesive layer. Then, it should be placed flat and enter the next process. During the subsequent production and storage process, attention should be paid to:

Avoid continuous flat placement: Prolonged flat placement may cause colloid flow, affecting the separation effect;

Prevent inversion: Inverting can cause the colloid to come into contact with the pipe plug, increasing the risk of contamination;

Standard stacking: Use dedicated trays or brackets to fix test tubes and maintain a vertical position.

Bubble elimination: centrifugal process optimization

Bubbles are a common issue that affects the quality of blood collection tubes and need to be treated through centrifugation technology

Centrifuge after vacuum plugging: It is recommended to leave the plug for 24 hours before centrifuging and packaging to ensure that the bubbles are fully visible;

Avoid centrifugation before plugging: Pre plugging centrifugation may cause colloidal displacement. It is recommended to check for bubbles 24 hours after plugging and re centrifuge if necessary;

Equipment selection: Prioritize the use of inclined centrifuges to improve defoaming efficiency.

If the blood collection tube needs to be sterilized by irradiation, the dose of cobalt 60 should be controlled to be ≤ 25kGy to avoid damage to the colloidal properties.

Hubei Xindesheng Material Technology Co., Ltd., as a professional enterprise in the field of blood collection tube additives such as serum separation gel, has always been committed to providing customers with high-quality products and comprehensive services. We not only have high-quality serum separation gel products, but also a full range of blood collection tube additives such as anticoagulants, coagulants, silicides, etc. If you have any related procurement needs in the near future, please click on the official website to learn more details!