The key role of TES buffer 7365-44-8 in free fatty acid (NEFA) determination kit

As the core index of fat metabolism, the detection accuracy of free fatty acid (NEFA) is of great significance for the clinical diagnosis of diabetes, cardiovascular disease and metabolic syndrome. In the NEFA assay kit, 2- [[tris (hydroxymethyl) methyl] amino] ethanesulfonic acid (TES buffer) is a core component that ensures the reliability of results by accurately regulating the pH value of the reaction system, optimizing enzyme activity, and improving detection stability.

1, Stable pH value: creating a "golden environment" for enzymatic reactions

NEFA determination involves multi-step enzymatic reactions, among which lipase catalyzed triglyceride hydrolysis and acyl CoA synthase activated free fatty acids are highly sensitive to pH values. TES buffer, with its unique molecular structure, forms a stable buffer system in the pH range of 6.8-8.2 through the synergistic effect of strong acidic groups of sulfonic acid groups and buffering groups of hydroxymethyl and amino groups. Experiments have shown that when the pH value of the reaction system deviates from the optimal range of enzymes, enzyme activity may decrease. TES buffer, on the other hand, controls pH fluctuations within an appropriate range by absorbing or releasing protons in real time, ensuring efficient enzymatic reactions.

TES buffer

2, Enzyme activity optimization: a catalyst that accelerates the reaction process

Acyl CoA synthase, as a key enzyme for NEFA determination, reaches its peak activity at pH 7.4. TES buffer improves the catalytic efficiency of the enzyme and significantly shortens the detection time by maintaining pH stability. In addition, the high purity characteristic (≥ 99%) of TES buffer effectively avoids the inhibitory effect of impurities on enzyme activity.

3, Anti interference capability: a "protective shield" against environmental fluctuations

Endogenous acid-base substances, temperature fluctuations, or transport bumps that may exist in clinical samples can interfere with the test results. TES buffer, with its excellent chemical stability, has a pKa value of 7.4 at 25 ℃, and its pH value is less affected by temperature, ensuring that the reagent kit can maintain stable performance in the range of 4 ℃ to 37 ℃.

4, Compatibility and safety: the cornerstone of ensuring the quality of reagent kits

TES buffer has good compatibility with other components in the NEFA kit, such as surfactants and preservatives, to avoid precipitation or loss of activity due to interactions between components. Its water-soluble number can quickly dissolve and form a transparent solution, simplifying the reagent preparation process. In addition, TES buffer does not participate in the reaction and has no toxic side effects, meeting the safety standards for clinical testing.

5, Clinical Application Value: The 'Behind the Scenes Hero' of Precision Medicine

In the management of diabetes, the level of NEFA is positively correlated with the degree of insulin resistance. TES buffer improves detection accuracy and helps doctors detect metabolic abnormalities in patients earlier.

Product packaging

Trimethylmethylaminoethanesulfonic acid (TES buffer) is a member of the Good's buffer family, with excellent buffering performance and a wide range of applications. Hubei Xindesheng Material Technology Co., Ltd. is a high-quality manufacturer specializing in the research and production of biological buffering agents, with rich production experience and advanced research and development technology. If you have any relevant needs, please click on the official website to learn more details or contact me directly!