Bicine buffer 150-25-4: performance advantages and application value in enzyme reaction experiments

In biochemical research, enzyme reaction experiments are the core of analyzing metabolic mechanisms and developing biocatalytic technologies. The activity and stability of enzymes are easily affected by the environment, and buffering agents, as the key to maintaining system stability, directly determine the reliability of experimental results. Bicine buffer, with its unique chemical properties, performs well in enzyme reaction experiments and has become a preferred choice for scientific research.

1, Accurately regulate pH environment to ensure stable enzyme activity

The catalytic activity of enzymes is highly sensitive to pH, and the active center amino acid residues need to bind to the substrate in a specific dissociation state. The dissociation state of the imidazole group in the active center of a protease, such as histidine, changes with pH. Overacidity or alkalinity can disrupt the conformation of the active center, leading to enzyme inactivation.

Bicine powder

The advantage of Bicine lies in its effective buffering range of 7.6-9.0, covering the optimal pH range for most enzymes such as oxidoreductases and transferases. Even if there are pH fluctuations due to substrate consumption or product generation during the reaction, Bicine can be rapidly regulated through proton acceptance and release. For example, when alkaline phosphatase catalyzes the hydrolysis of phosphate esters, it generates trace amounts of hydrogen ions, leading to a decrease in pH. Bicine can bind hydrogen ions in a timely manner to avoid pH deviation from the optimal value (about 8.0), ensuring the efficient activity of the enzyme.

2, Low interaction characteristics, guarding the structure and function of enzymes

The function of enzymes depends on specific spatial structures, and some buffering agents may disrupt enzyme conformation through electrostatic binding, hydrophobic interactions, etc. Amino buffering agents may form electrostatic bonds with the carboxyl group of the enzyme's active center, while high concentrations of phosphate buffering agents may bind to metal ions in the enzyme molecule, leading to enzyme inactivation.

The dihydroxyethyl group of Bicine molecule has good hydrophilicity, smooth charge distribution, and extremely weak interaction with enzymes. Experiments have shown that at conventional concentrations, Bicine has no significant effect on the secondary and tertiary structures of most enzymes. In the lactate dehydrogenase catalyzed lactate oxidation reaction, the enzyme specific activity difference between the Bicine buffered system and the non buffered control is less than 5%, while the activity may decrease by 15% -20% when Tris buffer is used, fully demonstrating its protective effect.

3, Excellent chemical stability, avoiding experimental interference

The experimental conditions for enzyme reactions are complex, and some require environments with coenzymes or metal ions at 37-55 ℃, with strict requirements for buffer stability. If the buffering agent decomposes, oxidizes, or reacts with other substances, it will lose its buffering capacity and generate interfering substances, which will affect the experimental results.

Bicine has excellent chemical stability and good tolerance under common experimental conditions. On the one hand, the molecular structure is stable, and on the other hand, it does not react with coenzymes, metal ions, or substrates, and does not interfere with the generation of impurities. In ATP dependent kinase reactions, Bicine does not undergo phosphorylation with ATP, nor does it bind to the necessary Mg ² ⁺ for the kinase, ensuring the purity of the system and guaranteeing experimental accuracy.

In summary, Bicine plays an irreplaceable role in enzyme reaction experiments due to its precise pH regulation, low enzyme interference, and high chemical stability. Whether it is enzyme kinetics research or biocatalysis experiments, it can provide a stable and suitable environment for enzymes, help researchers obtain reliable data, and promote the development of the field of biochemistry.

Product packaging

As a manufacturer of biological buffering agents, Desheng not only has rich experience in research and development, but also provides high-quality Bicine powder at a cheap price, all of which are of analytical grade and suitable for various biochemical experiments. At present, there is sufficient stock of spot goods, suitable for large-scale procurement. If you have any relevant intentions, please click on the website to inquire about details and purchase!